JCS -- Gartsbein et al. 119 (3): 470 Figure IG2

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Fig. 2. Protein kinase C ${delta}$ (PKC ${delta}$ ) specifically associates with STAT3. Keratinocytes (5 day cultures) were infected with isoform-specific PKC ${alpha}$ , ${delta}$ , ${eta}$ and ${zeta}$ recombinant adenoviruses, or with adenovirus encoding ß-galactosidase (ß-Gal) as a control, for 1 hour. OE, overexpression. Following infection, cells were incubated for 24 hours, then extracted and immunoprecipitated (IP) with antibodies against PKC ${alpha}$ , PKC ${delta}$ , PKC ${eta}$ and PKC ${zeta}$ . Immunoprecipitates were subjected to western blot analysis using isoform-specific anti-PKCs or anti-STAT3 antibodies. Results presented are representative of at least three experiments.