Fig. 7. JAM-A enhances sustained MAPK activation. (A) Immunoblot of phospho-ERK1/2 activity from serum-starved mock-transfected and JAM-A-overexpressing HUVEC samples plated on vitronectin for up to 60 minutes. Corresponding total ERK1/2 amounts are shown to indicate equal loading. (B) Densitometric analysis of normalized data from A, presented as mean ± s.e.m. (C) Immunoblot of phospho-ERK1/2 activity from serum-starved mock-transfected HUVECs, and JAM-A- and -257-overexpressing HUVEC samples plated on vitronectin for various times as indicated. Corresponding total ERK1/2 amounts are shown to indicate equal loading. (D) Densitometric analysis of normalized data from C, presented as mean ± s.e.m. Data shown are representative of at least three independent experiments.