Fig. 4. Nuclear dynamics in Slk19-deficient cells. cdc20 (US4235) and cdc20 slk19 (US4260) with integrated HTA2-EGFP were arrested in G1 with factor and released into YEPD medium. The cells were immobilized onto gelatin-coated glass slides containing 2% glucose in low immunofluorescence yeast nitrogen base with complete drop-out medium supplemented with adenine. GFP signals were sampled every 4 minutes. For each time point, seven Z-sections (0.5 µm apart) were taken and Z projections of these planes were made using Metamorph software.