Fig. 5. HA is a high-affinity ligand for GPR37 expressed in frog oocytes. (A) Currents induced by 100 nM HA were recorded from Xenopus oocytes injected with cRNAs coding for GPR37 and for GIRK1/2. Stimulation with medium served as control. (B) The current increase was dependent on HA concentration. Dose-response curves for a HA-induced increase in GIRK1/2-mediated inward currents were normalised against maximal currents obtained for each oocyte. Current increases were averaged over four oocytes prepared and injected on the same day. The values represent means ± s.d. Data are representative of several independent experiments.