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Figure 1


Fig. 1. Cellular localization of YFP-tagged myosin-IE. (A) Myosin-IE is preferentially localized at dynamic actin structures. Colocalization of YFP-Myosin-IE with actin demonstrated by epifluorescence microscopy. Actin was stained with TRITC-Phalloidin. YFP-Myosin-IE is shown in green, actin in red. (B) Localization of YFP-Myosin-IE (green) and coronin (red) at crown like structures as visualized by confocal microscopy. (C) Confocal analysis of the 3D distribution of YFP-Myosin-IE (green) and coronin. The X-Z and Y-Z views show myosin-IE predominantly at the side walls of large invaginations of the plasma membrane. Coronin localizes to the bottom of the invaginations and their upper rim. Bars, 10 µm (A,B); 3 µm (C).