Fig. 5. EphB2 activation promotes cell attachment to Matrigel-coated surfaces. Medullary tubule cells were plated on wells coated with Matrigel alone or together with ephrin-B1–Fc (0.3 µg/cm²) and allowed to adhere for 40 minutes. Ephrin-B1–Fc (2.0 µg/ml) or human Fc (2.0 µg/ml) with or without crosslinking with anti-human Fc antibodies (0.5 µg/ml) was also added in soluble form at the time of plating. Cell attachment measurements from three independent experiments are shown as mean ± s.d. Values were normalized to the control (stimulation with human Fc). Comparisons were performed using one-way factorial ANOVA. Exogenous ephrin-B1–Fc significantly stimulates cell attachment (P=0.004).