Fig. 6. Rearrangement of focal adhesions in medullary tubule cells is induced by treatment with ephrin-B1Fc but not EphB2-Fc. Cells plated on Matrigel were stimulated with 1 µg/ml ephrin-B1Fc, EphB2-Fc or human Fc for 15 minutes and stained with an anti-vinculin antibody. Focal adhesions revealed by vinculin staining are much more prominent in the cells stimulated with ephrin-B1Fc, but remain unchanged in the cells stimulated with EphB2-Fc. Left panels, lower magnification; right panels, higher magnification.