Fig. 2. PtdIns4KII
is a component of highly dynamic membranes. (A) A confocal image of a live GFP-PtdIns4KII
-expressing HT1080 cell. (B) Frames from a time-series experiment were projected in the Z-axis to reveal the paths of moving vesicles. These paths and the direction of movement (arrows) are detailed in C and D. (E) Frames from the same cell showing the progress of a vesicle (arrows) along a tubule toward a multivesicular body (also see supplementary material Movie 1). Bar, 10 µm.