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Figure 3


Fig. 3. GFP-PtdIns4KII{alpha} colocalises with endocytosed Rh-EGF. (A) Time course of Rh-EGF internalisation in HT1080 GFP-PtdIns4KII{alpha} cells. Detail from each time point is shown in the zoom boxes (10 µm square). (B) Anti-GFP western analysis of total cell lysates from HT1080 GFP-PtdIns4KII{alpha} cells stimulated with EGF for the times shown was also performed to rule out the possibility that the endosomal localisation could be due to degradation of the fusion protein. Bars, 10 µm.