Fig. 5. Inhibition of endogenous PtdIns4KII activity results in abnormal trafficking of internalised EGF. HT1080 cells were transfected with control monoclonal antibody 9E10 (A) or 4C5G (B) and, after serum starvation, stimulated with Rh-EGF then fixed at the time points indicated. All images are extended focus views representing 12 confocal sections collected 0.12 µm apart. Bars, 10 µm.