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Figure 6


Fig. 6. Inhibition of PtdIns4KII{alpha} mRNA expression results in the abnormal trafficking of internalised EGF. (A) siRNA with two independent duplex oligonucleotides (oligo-1 and oligo-2) effectively depletes HT1080 cells of PtdIns4KII{alpha} but not the closely related isoform PtdIns4KII{alpha} (the doublet in the PtdIns4KII{alpha} blot is frequently observed). A time course of Rh-EGF internalisation in HT1080 cells in mock (B) or PtdIns4KII{alpha} siRNA (C) transfected cells. (D) Detail of the boxed sub-plasma membrane region from the 900 seconds time point from C. Arrows indicate the retention of Rh-EGF in sub-plasma membrane punctae. All images are extended focus views representing 12 confocal sections collected 0.12 µm apart. Bars, 10 µm.