Fig. 2. Dermal fibroblasts were starved of growth factors in DMEM for 16 hours as described. 1-2x10⁶ cells were either left un-treated or treated with 1 µM ß-AR agonist in DMEM for 5-60 minutes at 37°C. After treatment, cell lysates were prepared, electrophoresed on 10% polyacrylamide gels and transferred to membranes. Membranes were immunoblotted with either an anti-phospho ERK antibody or an anti-ERK antibody (A). Three blots from three separate experiments were scanned for P-ERK and densitometry performed using a gel plotting macro in NIH Image 1.62. Data was averaged, statistically analyzed and represented graphically (B). Values plotted are means ± s.e.m. (n=3). ^*P<0.01 between ß-AR agonist and controls (0). The data shown are representative of three independent experiments from three separate cell strains.