Fig. 7. Sterile coverslips were coated with collagen and cells plated in FM. Cells were untreated (A), treated with 1 µM ß-AR agonist for 15 minutes (B), treated with 50 µM sp-cAMP (C) or rp-cAMP (E) for 45 minutes or pre-treated with either 50 µM sp-cAMP (D) or rp-cAMP (F) for 30 minutes prior to the addition of 1 µM ß-AR agonist for 15 minutes in FM. Cells were fixed and stained for actin (green) and vinculin (red). Bar, 20 µM