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Figure 2


Fig. 2. Peroxisomes align with, and are dispersed along, microtubules in cultured fibroblasts. (A) Immunofluorescence microscopy of peroxisomes and microtubules in control HSFs (top) and wild-type MEFs (middle). Bottom panels show confocal laser-scanning microscopy of a HSF. Green, PEX14; red, {alpha}-tubulin. (B) HSFs (top panels) and wild-type MEFs (bottom panels) treated with 20 µM nocodazole for 20 hours. PEX14 (green); {alpha}-tubulin (red). (C) Mitochondria (green) and microtubules (red) detected using MitoTracker Green FM dye and {alpha}-tubulin antibody, respectively, in untreated (-NCZ, top panels) and nocodazole treated (+NCZ, bottom panels) HSFs. Bars, 20 µm (top and middle panel in A; and B and C); 5 µm (bottom panel in A).