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Figure 1


Fig. 1. In vitro interaction of Flag-APRO4 with Srcwt. (A) The various APRO4 mutants used are represented schematically and the amino-acid sequence of the C-terminal region of APRO4 (145-152) is also shown. Proline residues that were mutated to alanine are represented on the amino-acid sequence. (B, left panels) In vitro translated 35S-labelled Flag-APRO4, and X-APRO4{Delta}N associated with Src GST-SH3-SH2 whereas Flag-APRO4{Delta}C did not. (C, left panel) Flag-APRO4 interacted with Fyn SH3-SH2 domains but not with Lyn, Blk, and various GST-SH3-SH2 fusion proteins: GAP (GTPase activating protein), PLC (phospholipase C{gamma}). (B,C, right panels) Coomassie staining of SDS-PAGE of purified GST-fusion proteins. Input: 100% of the respective 35S-labelled proteins used in binding reactions; GST: GST-beads. Data are representative of three independent experiments.