Fig. 7. Inhibition of FGF-mediated PC12 cell differentiation by overexpression of APRO4. (A,B) PC12 cells were transiently transfected with either an empty control vector, Flag-APRO4, X-APRO4N, Flag-APRO4C, or Flag-APRO4 and the indicated expression plasmids. After 24 hours, cells were serum starved for 20 hours, then either left untreated or treated with FGF for 2 days and analysed for expression of the differentiation marker Tubulin-ßIII by immunoblotting (IB). Actin was used as an internal control to verify equal loading of proteins. Data are representative of three independent experiments.