Fig. 7. Analysis of the role of ROCK in anchorage of mitochondria. CV-1 cells were transfected with plasmids encoding Myc-ROCK-RB/PH(TT) (ROCK-DN) (A,B) or Myc-ROCK-CAT (C,D). Cells were fixed; transfected cells were visualized by staining with anti-Myc antibody, and F-actin was visualized by TRITC-phalloidin. Bar, 10 µm. (E) Quantification of mitochondrial motility in cells expressing EYFP-Mito alone or EYFP-Mito and indicated mutant proteins in microinjected cells before or after treatment with 30 µM Y-27632 (Y27632) for 30 minutes or with 5.0 µM LPA for 5 minutes. Live cells expressing ROCK mutants were recognized by the presence of labeled mitochondria. Values are the mean percentage of fast movements ± s.e.m.; P<0.05; n, number of cells and, in brackets, number of organelle movements.