Fig. 9. Expression of pEGFP-mDia-N3 in CV-1 cells affects the distribution of mitochondria caused by disruption of microtubules. Distribution of mitochondria (A) or F-actin (B) in cells that were either transfected with pEGFP-mDia-N3 (B) or co-transfected with pEYFP-Mito and pEGFP-mDia-N3 (A). After depolymerization of microtubules with nocodazole, cells were fixed in 4% formaldehyde. F-actin was stained with TRITC-phalloidin. Cell margins in A are indicated by dots. Transfected cells are shown by arrows. Bars, 10 µm.