Fig. 1. Localization of endogenous SMN and of FP-SMN in transfected cells. In both (A,B) COS cells and (D,E) type-I-SMA fibroblasts, immunolocalization of the (A,D) endogenous SMN and direct detection of (B,E) FP-SMN revealed that the protein distributes to cytoplasm and nucleoplasm, and concentrates in CBs. (C,F) Enhanced green fluorescent protein (eGFP) diffusely localized throughout the cells. (G) COS cells were double-labelled for SMN (green) and CB marker coilin, gem marker gemin2 (H) and snRNP marker TMG (I) (all red). Inserts show SMN colocalized with coilin, gemin2 and snRNP in CBs. Bars, 3 µm.