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Figure 5


Fig. 5. Accumulation of endogenous SMN, coilin and snRNPs in CBs of COS cells transiently transfected with SMN constructs. (A) Distribution of snRNPs in cells transfected with SMN mutants: SMN, SMN{Delta}N86, SMN{Delta}ex7 and SMNTudor (all green) were co-stained with anti-TMG snRNA antibodies (red). (B) Cells transfected with SMN mutants (green) were immunolabelled for endogenous SMN (red). (C) Distribution of coilin, TMG and U2 snRNP-specific B" protein in COS cells transfected with SMNE134K mutant. (D) Distribution of the U2 snRNP-specific B" protein in cells transiently transfected with SMN mutants. Inserts show magnified images of the distribution of those components in NBs of transfected cells. (E) Analyses presented in histograms. Bars, 3 µm.