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Figure 6


Fig. 6. Analyses of the interaction of the fluorescently tagged SMN mutants with endogenous SMN and snRNPs. (A) COS cells were transfected with FP-SMN or FP-SMN{Delta}ex7. Immunoblotting shows no significant degradation of gem/CB components in whole-cell extracts prepared from FACS-selected cells. (B-H) Cell extracts were immunoprecipitated with anti-GFP (GFP IP) or anti-TMG snRNA antibodies (TMG IP) and immunoblotted for endogenous SMN. Incubation with anti-GFP served as loading control. SMN mutants coimmunoprecipated the endogenous SMN, except for the Tudor mutant. Both FP-SMN and FP-SMN{Delta}ex7 coimmunoprecipitated with anti-TMG antibodies as confirmed with control IgG (DAKO IP). Light (lig) and heavy chains of immunoglobulins (hig).