Fig. 3. Dabp1 is enriched in the cortex and the leading edge of chemotaxing cells. Growing cells (top panels) and developing cells (bottom panels) were fixed and stained with affinity-purified anti-Dabp1 antibodies followed by BODIPY-FL-labeled secondary antibodies (to detect Dabp1) and TXRED-labeled phalloidin (to detect actin). Arrows and arrowhead show areas where Dabp1 and actin colocalize (in the merged images). Bar, 10 µm.