Fig. 4. (A) The cortical localization of Dabp1 requires an organized actin cytoskeleton. Wild-type cells were treated with 10 µM cytochalasin A for 40 minutes and then stained with affinity-purified anti-Dabp1 antibodies (Dabp1) and TXRED-labeled phalloidin (actin). (B) Neither the absence of, nor an increase in, Dabp1 protein influences the actin cortex. Wild-type cells (WT), Dabp1 null cells (Dabp1^-) and cells overexpressing Dabp1 (Dabp1⁺) were fixed and stained with TXRED-labeled phalloidin. Bar, 10 µm.