Fig. 6. Par-1 acts specifically in the Notch pathway. Embryos were injected with buffer (A,C,E) or par-1 dsRNA (B,D,F) before analysis. (A,B) Anti-Achaete staining of four segments at stage 9 shows one positive cell per proneural cluster in control, whereas many proneural cells are positive after par-1 RNAi treatment. (C,D) Stage 15 arm-GAL4 UAS-NECN embryos stained for Neuroglian show severe reduction in neural tissue. (E,F) Stage 10 embryos stained for Engrailed.