Fig. 2. Immunoblotting and immunofluorescence with an antibody against MAP65 peptide. Samples at equal protein loading immunoblotted with the antibody against the CEEESWLEDYNR peptide. (A) Immunoblot of Zinnia suspension cells at 0 hours (pre-induction); (B) differentiating cell culture at 48 hours. (C) Immunofluorescence of induced microtubule bundles at 48 hours. Bar, 10 µm.