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Figure 2


Fig. 2. Expression of the TGFß pathway during EB formation. (A) Human ES cells were allowed to spontaneously differentiate to form EBs. The expression of components of the TGFß pathway was examined by RT-PCR. GAPDH primers were used as loading control. (B) The expression of TGFBR2 in undifferentiated ES cells was examined in three independent human ES cell cultures in which the expression of POU5F1 (OCT4) was also verified. A negative control experiment (-) was performed using cDNA from MEFs. The ACVRL1 primers showed some cross-reactivity with mouse Acvrl1.