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Figure 3


Fig. 3. Effect of TGFß on EB morphology and expression of endothelial markers. Day 18 EBs treated with 1 ng/ml of TGFß for 18 days. Sections of (A) control and (B) TGFß-treated EBs were examined by H&E staining. Prominent cavities can be seen in both samples. The expression of (C) PECAM1 and (D) KDR was assessed by qRT-PCR, normalized to GAPDH. Results were presented relative to expression in untreated samples.