Fig. 9. (A) MMP-2 is activated upon PMA treatment. Cells lysates and supernatants from HUVECs treated with PMA for indicated times were analysed by gelatin zymography. The active form of MMP-2 (62 kDa) became apparent, revealing the processing of pro-MMP-2. One representative experiment out of four is shown. (B) Localisation of MMP-2 and MT1-MMP to podosomes. HUVECs were treated with PMA for 1 hour, fixed and stained with anti-MMP-2 or anti-MT1-MMP antibodies and rhodamine-phalloidin to visualise MMP-2 or MT1-MMP (green) and F-actin (red), respectively. Bar, 10 µm.