Fig. 2. Sec8 overexpression favors OL morphological differentiation. (A-D) OL progenitors were infected overnight with adenoviruses encoding (A) control EGFP (EGFP-AV) or (B-D) FLAG-tagged Sec8 (Sec8-AV), allowed to differentiate for 3 days, fixed, labeled with anti-MBP- (red) and anti-FLAG- (green) Abs and analyzed by epifluorescence microscopy. OLs were assigned to three morphological categories: (B) low, (C) medium or (D) high complexity. Cell bodies were overexposed in order to observe protein localization in the processes. Bars, 10 µm; px, pixels. (E) Mature MBP-positive cells were plotted as percentage of the total infected cells. (F) The number of OLs in each morphological category is shown as a percentage of the total MBP-positive OLs (500 cells/condition, n=6 from three independent cultures). (G) The areas of 100 randomly chosen MBP-positive cells per condition were measured and plotted. Only infected cells were analyzed. (E-G) White bars, control EGFP-AV; black bars, Sec8-AV infected.