JCS -- Park et al. 119 (5): 819 Figure IG7

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Fig. 7. Effects of APC transfection on RAS- and ß-catenin-induced foci of transformation in DLD-1 cells. 1x10⁴ DLD-1 cells were transfected with 0.5 µg each of different combinations of pCMV, pCMV-APC, pcDNA3.0-Flag-ß-catenin, or pMT3 RAS-L61, and selected with G418. After 12 days, the cells were stained with 0.5% crystal Violet in 20% ethanol and the foci photographed.