Fig. 8. Effects of APC on RAS-induced morphological transformation in NIH 3T3 cells. (A) NIH 3T3 cells containing doxycyclin (Dox)-inducible H-Ras (G12R) (Lim et al., 2004) were grown in DMEM containing 10% FBS, and were treated (or not) with 2 µg/ml Dox of for 24 hours. They were examined by phase-contrast microscopy, photographed (x200), and p-ERK, ERK, -tubulin and RAS were detected by western blotting. (B) RAS-inducible NIH 3T3 cells were grown as in A, and transfected with 0.5 µg of either pEGFP-C1 or pEGFP-C1-hAPC. Cells were visualized by fluorescence microscopy. Arrows indicate cells expressing APC-EGFP.