Fig. 7. The acidic cluster is required for transport of CsA-Rh50 from the plasma membrane to the CV. Cells expressing pFL775 (A) or pFL778 (B) were treated as described in Fig. 5. Mutation of the FW signal to alanine residues (pFL775) did not affect the transport of the chimera to the CV whereas mutation of the acidic cluster to alanine (pFL778) inhibited CV targeting. Bar, 10 µm.