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Figure 4


Fig. 4. Silencing of the Kazal1 gene in intact adult H. vulgaris. (A-E,H,I) Kazal1 expression was detected after whole-mount in situ hybridization with (A,B) NBT-BCIP and (C-E,H,I) Fast-Red. A decrease in the level of Kazal1 expression was observed after 2 to 5 exposures to Kazal1 dsRNA (A,C,D), but not in hydra treated with (B) unc22 dsRNA or (E) agarose. (F,G) Tsp1 expression was not altered in hydra exposed 5x to Kazal1 dsRNA (upper) when compared to untreated animals (lower). Confocal views showing the residual or undetectable level of Kazal1 expression in gland cells of hydra exposed 5x to Kazal1 dsRNA (I) when compared to control hydra treated with agarose (H). DAPI staining (blue), {alpha}-tubulin staining (green). Bars: 150 µm (C-E,F), 4 µm (H,I).