Fig. 5. Survival and budding rates of Kazal1(–) H. magnipapillata. (A) Sizes of the hydra populations exposed to Kazal1 dsRNAi. After each feeding hydra were counted and the percentage of Kazal1-silenced hydra over the control population exposed to the empty vector dsRNA was calculated. Each curve corresponds to a different experiment; 100, 65 and 60 hydra were initially taken for RNAi-a, RNAi-b and RNAi-c, respectively. (B) Budding rates observed in Kazal1(–) and control hydra populations in (a) RNAi-a, (b) RNAi-b and (c) RNAi-c experiments. The budding rate corresponds to the ratio between the number of new hydra produced by budding over the total number of hydra. Dark bars, control hydra; light bars, Kazal1(–) hydra. (C) Duplication of the peduncle and basal region observed in two different Kazal1(–) hydra after three feedings (RNAi-b).