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Figure 6


Fig. 6. Cellular disorganization of gland cells in Kazal1(–) intact hydra. (A-F) Confocal images of H. magnipapillata gland cells exposed up to 7x either to (A,D,F) Kazal1 or to (B,C,E) empty vector dsRNAs, subsequently immunodetected with (A,B) anti-RSK, (C,D) anti-{alpha}–tubulin, (E,F) 6C4 anti-LBPA antibodies (green) and stained with (E,F) Hoechst 33342 dye (blue) and the mitotracker dye MitoFluor Red 589. (F) Anomalies from four distinct gland cells; arrows indicate fused vacuoles, arrowheads cytoplasmic portions that are expelled from the cell. Bars, 10 µm (A-D), 5 µm (E,F). (G) Evolution of the gland-cell index over the course of three independent RNAi experiments (RNAi-a, -b, -c). For each condition, the percentage of gland cells over doublets of dividing interstitial cells was measured in control and Kazal1(–) hydra. This percentage was stable in control hydra with a 90% to 100% value.