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Figure 8


Fig. 8. Kazal1 silencing in regenerating hydra. (A-C) Kazal1 expression in wild-type regenerating hydra fixed 4, 6 and 30 hours after mid-gastric section. Notice the strong level of expression in the endodermal cells of the (A,B, arrowheads) foot-regenerating tips and (A,B,C, arrows) head-regenerating tips. (D,E) Kazal1 expression in H. magnipapillata having regenerated 4 hours is similar to the wild-type pattern in control hydra exposed 6x to (D) empty vector but strongly silenced after 6x exposures to (E) Kazal1 dsRNAs. The arrow and arrowhead indicate the residual expression in head- and foot-regenerating tips, respectively. (F) In intact hydra 4x exposures to Kazal1 dsRNAs (right) suffice to completely silence Kazal1 expression when compared with the control hydra exposed to empty vector (left). Bars, 150 µm. (G) Kinetics of tentacle rudiments appearance were similar in regenerating H. magnipapillata exposed 3x to unc-22 ({square}) or to Kazal1 ({diamondsuit}) dsRNAs. (H) Appearance of tentacle rudiments recorded 42 hours (left) and 44 hours (right) after mid-gastric section of hydra exposed up to 9x either to empty vector ({square}) or to the Kazal1 ({diamondsuit}) dsRNAs.