Fig. 8. Dynamic changes of fluorescence intensity ratio and of FRET of paxillin-dSH2 and FAK-dSH2 pairs. The fluorescence measurements were taken at 1-minute intervals. The color scales on the right indicate the fluorescence ratio or FI values. Note that the temporal variation in the ratio of both donor/acceptor pairs is relatively stable. FI values for the FAK-SH2 pair showed some local fluctuations, whereas high values for paxillin-dSH2, mostly restricted to areas with high PY remained stable over time. Bar, 2 µm.