Fig. 1. The bem1-3 growth defect is suppressed by both SEC15 and SEC4 multi-copy plasmids. S. cerevisiae bem1-3 cells (NY1444) carrying 2 µ SEC15, 2 µ SEC4 or 2 µ BEM1 plasmids (pNB192, pNB142 and pNB1227, respectively) were spotted onto YPD plates along with bem1-3 cells carrying no plasmid. The plates were incubated at 25°C, 30°C, 34°C and 37°C for 3 days and scanned.