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Figure 8


Fig. 8. Sec15-1–GFP mislocalizes as does Sec8-GFP in a sec15-1 background. (A) GFP-tagged sec15-1 (sec15-1 GFP; NY2565) or SEC15 (Sec15 GFP; NY2557) cells were shifted to restrictive temperature (37°C) or grown at permissive temperature (25°C) for 1.5 hours and processed for GFP fluorescence detection. Cells were visualized by direct fluorescence microscopy. (B) The proportion of cells containing a polarized GFP signal from the experiment shown in panel A was scored. At least 200 cells were scored in each strain. (C) Cells expressing GFP-tagged Sec8 (Sec8GFP), either in a wild-type (NY2558) or sec15-1 (sec15-1; NY2566) background were shifted to restrictive (37°C) or grown at permissive (25°C) temperature for 1.5 hours and processed for the direct GFP fluorescence detection. The localization was visualized by direct fluorescence microscopy. (D) The proportion of cells containing a polarized GFP signal from the experiment shown in panel C was scored. At least 200 cells were scored in each strain.