JCS -- Baudry et al. 119 (5): 889 Figure IG3

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Fig. 3. Ectopic expression of PKB ${alpha}$ by retrovirus infection restores adipogenesis in PKB ${alpha}$ ^–/– MEFs. (A) PKB ${alpha}$ ^–/– MEFs were infected with retrovirus expressing HA-PKB ${alpha}$ or vector only. After puromycin selection, extracts of cells were analysed by western blotting for the expression of HA-PKB ${alpha}$ . (B) Cells were exposed to the adipogenic cocktail and stained with Oil Red O after 15 days. (C) RT-PCR analysis of adipose markers PPAR ${gamma}$ and aP2 in PKB ${alpha}$ ^–/– MEFs infected with vector or HA-PKB ${alpha}$ -expressing retrovirus and submitted to adipose differentiation.