Fig. 4. PTEN is enriched in the axon of DRG neurons where it accumulates with microtubules. (A) A single confocal section taken through an E18 trunk of a rat embryo labelled with anti-ßIII-tubulin (left) and anti-PTEN (middle) antibodies. PTEN is present in the nucleus of DRG neurons (^*) and the surrounding cytosol and in the axons of the peripheral projection of the DRGs (arrowhead). (B) Rat E18 DRG neurons were triple-labelled with anti-ßIII-tubulin (left), anti-PTEN (middle) antibodies and phalloidin (blue channel, right). In the growth cone, PTEN colocalises with the microtubule in the C-domain (arrow), whereas little signal is detected in the P-domain or the growth cone membrane (arrowhead). Bars, 10 µm.