Fig. 5. PTEN and GFP-PTEN are enriched at microtubules in N1E-115 cells. (A) NIE-115 cells were labelled with anti-ßIII-tubulin (left) and anti-PTEN (middle) antibodies. Similarly to primary DRG neurons, there is an accumulation of endogenous PTEN with microtubules in N1E-115 cells. (B,C) NIE-115 cells were transfected with a control siRNA (B) or PTEN specific siRNA (C), and labelled with the anti-PTEN antibody (left) and Phalloidin to visualise F-actin. Confocal micrographs, taken with identical scanning parameters, demonstrate the loss of PTEN signal in the PTEN siRNA-treated cells. (D) Similarly to the distribution of endogenous PTEN, GFP-PTEN accumulates with microtubules in N1E-115 cells. Bars, 10 µm.