Fig. 8. Upregulation of CT-1 mediated by menadione and CoCl₂ is inhibited by the HIF-1 inhibitor 2-ME (3 µM). Embryoid bodies were treated at day 4 of cell culture with either menadione (20 µM) or CoCl₂ in the presence of 2-ME. CT-1 protein expression was assessed after 24 hours by semiquantitative immunohistochemistry. ^*P<0.05, significantly different from levels in the untreated control; ^#P<0.05, significantly different to levels before addition of 2-ME.