Fig. 8. Wortmannin disrupts the association of Varp protein with the early endosomes. HeLa cells expressing Varp were treated with DMSO or 100 nM wortmannin for 30 minutes at 37°C. The cells were fixed and stained with anti-Varp (red) and anti-EEA1 (green) antibodies. Bars, 10 µm.