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Figure 7


Fig. 7. Sequential re-entry of Sp1, Sp3 and RNAP II into daughter nuclei. MCF-7 cells were grown on coverslips in estrogen-complete medium, fixed, and double labeled with anti-Sp1 or anti-Sp3 and anti-RNAP II antibodies or with anti-Sp1 and anti-Sp3 antibodies. DNA was stained by DAPI. Spatial distribution was visualized by fluorescence microscopy in the ApoTome mode. Bars, 5 µm.