Fig. 8. Mitotic colocalization of Sp1 with F-actin. MCF-7 cells were grown on coverslips in estrogen-complete medium, fixed, and labeled with rabbit polyclonal anti-Sp1 antibodies and mouse monoclonal anti-calnexin or anti-cytokeratin 8 or anti-cytokeratin 18 antibodies. F-actin was labeled by Alexa-Fluor-488-conjugated phalloidin. DNA was stained by DAPI. Spatial distribution was visualized by fluorescence microscopy and image deconvolution as described in Materials and Methods. Single optical sections are shown. Yellow in the merge images signifies colocalization. Bar, 5 µm.