Fig. 1. Protein components of melanosomes. (A,B) Early melanosomes and CCV isolated from M14 or MNT-1 cells were analyzed by IB with antibodies against Pmel17, AP1, AP2 and TfR, as indicated. Experiments were performed twice with comparable results. (C) Pmel17 was immunoprecipitated using PEP13h and was then immunoblotted with antibodies as noted. (D) Melanoma cells as noted were fixed and double stained for Pmel17 (red) and AP1 or AP2 (green) as indicated. Representative images for each combination are shown. Bars, 10 µm.