Fig. 7. Detection of µ1B by TISH in the skin. Skin biopsies were examined by TISH as reported in the Methods. (A,B) Skin before UV stimulation shows Pmel17 and µ1B expression in melanocytes (black arrows) in the epidermis, respectively. (C) Skin 1 day after UV stimulation, shows a moderate increase in the number of µ1B-positive cells and intensity of µ1B expression. (D) Staining with sense probe showed no specific staining. (E,F) Specific expression of µ1B in the cytoplasm of polarized epithelial cells in the skin (sebaceous glands) and kidney (tubular cells) as positive controls. Bars, 100 µm.