Fig. 1. Activities of mutant forms of Fmi under various developmental contexts. (Left) Schematic representation of the Fmi protein (3575 aa long) and various mutant forms designed by us. The extracellular region includes eight tandemly repeated cadherin repeats (Cadherin), five EGF-like domains (EGF-like), two laminin G domains (Laminin G), and a hormone receptor domain (HRM). (Right) Results corresponding to the mutant forms shown on the left. `Rescue from lethality' indicates whether the lethality of fmi-null mutants was prevented by expression of either form by using the pan-neuronal driver Gal4-1407 or not. Relevant genotypes were fmiE45 Gal4-1407/fmiE59; UAS-transgene/+. Relevant genotypes of `rescue from overgrowth phenotype', `overexpression in all da', `rescue of overlap phenotype' and `overexpression in ddaC' were as described in the legends of Figs 2, 3, 4, 5, 6, respectively. Data from the `cell aggregation assay' are shown in Fig. 8. `Effect of overexpression on PCP' indicates effects of overexpression on reorientation of wing hairs, as shown in Fig. 9A-D. +, rescue or formation of cell aggregates; - rescue did not occur or cells did not aggregate. Fmi and Fz, effect of overexpression of individual forms resembling that of Fmi and Fz overexpression, respectively (see details in Fig. 9A-D). N.D., not determined. Inactivity of 
HR::EYFP and CR:EYFP was reminiscent of results of in vivo structure-function analysis of DE-cadehrin (Oda and Tsukita, 1999), and we discussed this result and other constructs in Materials and Methods.