Fig. 3. Localization of Cdc14p-YFP expressed from its endogenous promoter. (A) Cdc14p is not tethered to the nucleolus during interphase. Exponentially growing cells expressing CDC14-YFP NOP1-CFP (JC252) were prepared for microscopy. Images of Cdc14p-YFP, Nop1p-CFP (blue) plus DAPI (red) and the merge of the three channels plus DIC (Merge+DIC) are shown. Representative photographs with different cell-cycle stages are shown (see text). (B) Cdc14p is located at the SPB. Exponentially growing cells expressing CDC14-YFP/CDC14-YFP (JC189) were grown in YPD at 25°C and scored for Cdc14p-YFP at foci (n>100; representative photographs with faint and strong foci signal are shown). (C) Micrograph of a cell in anaphase from strain JC310 (CDC14-YFP TUB2-CFP). (D) Cdc14p is localized to the bud neck in a fraction (35% of binucleated cells) of large budded cells (JC189). (E) Quantification of the localization of Cdc14p at the SPB at different stages of the cell cycle from the experiment shown in B. Bars, 2 µm.